CALLUS
INDUCTION OF BRINJAL BY GENOTYPE AND GROWTH REGULATORS
M. A. Alim,
B. K. Biswas, M. Hasanuzzaman, Pronay Bala*and
U. K. Roy
*Corresponding Author; E-mail:kbdpronay@yahoo.com
Abstract
The
investigation was carried out in Tissue Culture Laboratory of the Department of
Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology
University, Dinajpur during the period from September, 2011 to January, 2012
with a view to study the effect of genotypes and growth regulators on callus induction
of Brinjal (Solanum sp.). Three
genotypes of Solanum viz. Protab,
Green Ball and Ghemma Begun (Local) were used to assess their regeneration
ability. Leaf segments of three genotypes of Solanum were
cultured on MS medium with different concentrations and combinations of
hormones. Callus induction was ranged from 66.00 to 82.67% among the genotypes,
Protab showed early callusing (7.533 days) with maximum rate of callus
induction (82.67%). The highest percentage of callusing appeared in MS + 3 mg L-1
2,4-D + 0.05 mg L-1 BAP
considering all genotypes. Based on the overall performance, the genotype
Protab appeared as the best for number of callus initiation (16.53).
Key words: Callus
induction, Brinjal genotype and Growth regulators.
Download PDF: Click here
Return Volume: Journal of Experimental Biosciences 5(2): July 2014
