ANTIBACTERIAL
EFFECT OF NEEM (AZADIRACHTA INDICA) LEAF EXTRACT IN ETHANOL (NLEE)
AGAINST STAPHYLOCOCCUS AUREUS, ESCHERICHIA COLI AND PSEUDOMONAS
AERUGINOSA
Shamima Sultana, ASM Shahidullah,
Md. Mahbubul Islam*, A.F.S.A. Wasey, Shamsun Nahar and S.A. Nasrin
*Corresponding author;
Email: mahbub_agronomy@yahoo.com
Abstract
The study was conducted during the
period from July 2004 to June 2005 in the Department of Pharmacology and
Therapeutics in collaboration with Department of Microbiology, Mymensingh
Medical College to determine the profile of antibacterial effect of crude neem
leaf paste (CNLP), neem leaf extract in ethanol (NLEE), and standard antibiotic
Ceftriaxone against Staphylococcus aureus, Escherichia coli and Pseudomonas
aeruginosa. Four separate experiments were conducted. At first inhibitory
effect of crude neem leaf paste (CNLP) was determined incorporating CNLP into
nutrient agar media (NA), against Staphylococcus aureus, Escherichia coli and
Pseudomonas aeruginosa. Secondly minimum inhibitory concentration (MIC)
of neem leaf extract in ethanol (NLEE) was determined against the three test
organisms by broth dilution technique. And thirdly minimum inhibitory
concentration (MIC) of standard antibiotic ceftriaxcone against test organisms
was determined by broth dilution technique as well as making a comparison
between MICs of neem leaf extract in ethanol (NLEE) & antibiotic
Ceftriaxone. Subculture study of materials from effective CNLP, NLEE and
Ceftriaxone in nutrient agar medium was made for confirmation of respective
results of previous experiments I, II, III. Results revealed that inhibitory
effects were observed against the growth of S. aureus, E. coli and P.
aeruginosa at 15%, 20% and 25%, respectively of CNLP incorporated into NA
media. The broth dilution technique was followed to determine the MICs of NLEE
and Ceftriaxone. The MIC of NLEE was 666 μg/ml against S. aureus and
that against E. coli and P. aeruginosa was 1332 μg/ml. The MIC of
Ceftriaxone was 10μg/ml against S. aureus and that against E. coli and
P. aeruginosa was 25 μg/ml. The MIC of Ceftriaxone was the lowest in
comparison to MICs of NLEE. The subculture study showed similar results with
that of previous experiments in terms of inhibitory effects of CNLP and MICs of
NLEE & Ceftriaxone against all of the organisms studied.
Keywards: Antibacterial
effect, crude neem leaf paste, Neeem leaf extract in ethanol, Ceftraxione
(antibiotic), Minimum inhibitory concentration, Staphylococcus aureus,
Escherichia coli, Pseudomonas aeruginosa.
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Return Volume: Journal of
Experimental Biosciences 6(1): January 2015
