COMPARISON OF ESTERASE ACTIVITY BETWEEN THE FOURTH INSTAR LARVA AND PUPAL STAGE OF CHIRONOMUS SPECIES IN BANGLADESH

Author (s): M. M. Ahasan1*, F. Rozy2, M. A. Rahman2, M. Hasan1 and A. F. M. Jamal Uddin3
*For corresponding, E-mail: labu63@gmail.com

 Abstract

Comparisons of esterase activity between the 4th instar larva and pupal stage of Chironomid species were made at the Molecular Biology Laboratory, University of Dhaka during 2008 using 7.5% Polyacrylamide gel electrophoresis (PAGE). The staining substrates were ╬▒ and ╬▓naphthyl acetates. The study on Chironomid species was conducted during January to December, 2008 in the Genetics & Molecular Biology, Department of Zoology, University of Dhaka, Bangladesh. Esterase Zymograms were prepared from 4th instar larva and pupa. Chironomid larvae showed a stage-specific variability in esterase expression. The maximum four esterases were assigned in this experiment Est-1, Est-2, Est-3 and Est-4 with different relative mobilities. But these four esterases were detected in 4th instar larva. Pupa possessed only one esterase (Est-1).

Key words: Protein polymorphism, Electrophoresis, Chironomid larva, Pupal stage.




J. Expt. Biosci. 2(1): January 2011


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