IN VITRO ANTIOXIDANT ACTIVITY OF THE METHANOLIC EXTRACTS OF LEAF AND FRUIT OF CALAMUS ROTANG LINN.

Author (s): Aparna Shil1*, Mahbubul Kabir Himel1, Abul Khair1, M. Nur Alam2 and A.F.M. Jamal Uddin3
* Corresponding author, E-mail:aparna@juniv.edu

 Abstract

Plants can be the primary source of various traditional medicines that play an important role in health care. The present study was designed to investigate the antioxidant activity of the crude methanolic extracts of leaves and fruits of Calamus rotang Linn. using four in vitro methods namely, total Antioxidant activity, Flavonoid and phenol content and DPPH scavenging activity of the sample plant extract. Gallic acid equivalent phenolic content was highest in the fruit extract of the Calamus rotang. On the other hand, quercetin equivalent flavonoid content was highest in the leaf extract of the plant. The IC50 value of leaf and fruit obtained by DPPH method was comparable to that of standard antioxidant. These were 387.95 µg/ml and 142.01µg/ml for leaf and fruit, respectively whereas ascorbic acid showed the value of 25.826 µg/ml, representing a considerable choice of option for further studies. However, leaf extract of C. rotang showed highest antioxidant activity irrespective of the methods used.

Key words: Antioxidant, Flavonoid content, Phenolics content and IC50 value



J. Expt. Biosci. 3(2): July 2012


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