Author (s): T. A. Poly1, H. Huq2*, M. E. Hoque3 and M. S. Alam4
* Corresponding author, E-mail:


The study was undertaken to develop an efficient protocol for in vitro regeneration of garlic (Allium sativum). One exotic designated as G1 and three local garlic varieties designated as G5, G8 and G10 were used as experimental materials where root tips, basal discs and leaf discs were used as explants. MS (Murashige and Skoog) medium supplemented with different concentrations and combinations of 2,4-D and BAP were used for callus induction and regeneration of plantlet. Maximum callus induction was observed in genotype G8 (69.41%) and explant leaf disc (72.74%). In combined effect both genotypes G1 and G5 showed highest callus induction (86.63%) from leaf discs placed in medium supplemented with 2 mg/1 2,4-D. Among the treatments MS + 1.5 mg/1 2,4-D and 0.5 mg/1 BAP showed the best performances in callus proliferation as well as maintenance of calli. MS medium supplemented with 10 mg/1 BAP showed higher percentage of shoot regeneration (61.08%) and numbers of shoots per callus (14.22). G5 showed the best performance for induction of shoots (62.93%). Percent shoot regeneration was highest (58.86%) in leaf disc. Among the local varieties, G5 showed the best performance and among the explants used, leaf disc was found best. The survival rate of transferred regenerated plantlets was quite satisfactory (80%).
Key words: In vitro, Garlic, MS medium and Callus.

J. Expt. Biosci. 3(2): July 2012

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