BIOCHEMICAL
CHANGES IN THE VIRUS INFECTED PUMPKIN PLANTS
Fatema
Begum*, A. M. Akanda, M. A. T. Masud
*Corresponding author;
Email: fatema22_sau@yahoo.com
Abstract
Twenty six pumpkin lines were
evaluated against different viruses. Four viruses were identified by DAS-ELISA
namely Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), Watermelon
mosaic virus 2 (WMV2) and Zucchini Yellow mosaic virus (ZYMV) and symptoms were
present in field but antisera were not used, identified as other potyvirus.
After detection the present experiment were conducted to determine the
biochemical changes in Cucurbita moschata due to infection of different
viruses. The cellular components were varying extent in infected pumpkin leaves
compared to healthy ones. Chlorophyll a, Chlorophyll b and β-carotene were
decreased in virus infected leaves compared to healthy ones. The ratio of
Chlorophyll a and b was decreased due to virus infection in all cases. The
highest reduction of Chlorophyll a, Chlorophyll b and β-carotene was observed
in CMV infected leaf. The lowest reduction of Chlorophyll a, Chlorophyll b and β-carotene
was observed due to PRSV-W infection and no change was observed in healthy
leaves. There was positive and significant relationship between SPAD value and
yield of pumpkin plants.
Keywords: Biochemical
changes, Five viruses (PRSV-W, CMV, ZYMV, WMV2 and other potyvirus), Pumpkin.
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Return Volume: Journal of
Experimental Biosciences 6(1): January 2015
