CALLUS INDUCTION ABILITY OF INTROGRESSED BRASSICA GENOTYPES UNDER VARYING SALT STRESS CONDITIONS
Authors: Mst. Rowshanara,
Nighat Sultana, Md. Asadur Rahman, Lutfur Rahman
and K. M. Nasiruddin5
Corresponding
author:
rowshan.lima82@gamil.com (Mst. Rowshanara)
Abstract
The
experiment was conducted at the Biotechnology Laboratory, Department of
Biotechnology, Bangladesh Agricultural University, Mymensingh, during January
to May, 2007 to determine the callus induction
ability of five genotypes of Brassica at different levels of salt stress set imposing varied concentrations
(0, 0.3, 0.6 and 0.9 NaCl) supplemented with 1 mg/1 2, 4-D and 10 mg/1
BAP and 3 mg/1 AgNO3. Shoot tips of five
Brassica genotypes (G26, G16,
G25, G17 and G24) were cultured in MS media
having the varied salt concentrations. Results showed that the percent callus
induction of different genotypes varied in different treatments in respect of
susceptibility to NaCl. In general, callus weight and size decreased with the
increase of salinity level. Among the genotypes and G26 showed more
callus induction ability than the other genotypes.
Key words: Introgressed, Brassica, Callus induction, Salt stress
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